The Flow Cytometry Lab is housed in the Department of Immunology, Rheumatology and Allergy, Medical University of Lodz, Room 61 and is operated by Joanna Makowska, PhD, MD and Aleksandra Piechota-Polanczyk, PhD.
The FC Lab has analytical cytometer (FACS LSR Fortessa II with Diva software operating system). The FACS LSR Fortessa II has five lasers and can analyze cells based on 17 spare parameters (side and forward side scatter and 15 different fluorescent colors). It has as well a HTS tray which enables to measure 96 samples form 96-well plate at once.
The FC Lab is using protocols for immunophenotyping of peripheral blood cells and cells suspensions form human epithelial cells. Flow cytometric phenotyping is used diagnostically to determine the cell type of lymphocytes, activity of leucocytes (phagoburst), therapeutically to monitor cell populations, and in research to monitor cell populations following infection, disease course and immunization studies (basotest). Moreover, we are using FC to determine cell viability and to detect intra- and extracellular cytokine and interleukin production.